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KMID : 1132720130110010055
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Genomics & Informatics
2013 Volume.11 No. 1 p.55 ~ p.57
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siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens
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Singh Nitin Kumar
Seo Bo-Yeun
Vidyasagar Mathukumalli
White Michael A.
Kim Hyun-Seok
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Abstract
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Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets. As multiple sources of variations that are unique to siRNA screens exist, there is a growing demand for a computational tool that generates normalized values and standardized scores. However, only a few tools have been available so far with limited usability. Here, we present siMacro, a fast and easy-to-use Microsoft Office Excel-based tool with a graphic user interface, designed to process single-condition or two-condition synthetic screen datasets. siMacro normalizes position and batch effects, censors outlier samples, and calculates Z-scores and robust Z-scores, with a spreadsheet output of >120,000 samples in under 1 minute.
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KEYWORD
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high-throughput screening assays, RNA interference
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